Qalabka H.pylori IgG ELISA

Xirmadu waxay isticmaashaa hab aan toos ahayn ELISA si ay u ogaato unugyada difaaca ee Cag-A (nooca I) iyo Hsp-58 (nooca II) antigens ee Helicobacter pylori (HP) ee serum ama balasmaha.Saxanka falcelinta microtiter waxaa lagu dahaadhay tibaaxaha hidde ahaan la safeeyey ee antigens-ka sare, kaas oo si gaar ah ugu xidha unugyada difaaca ee serum si loo tijaabiyo, ka dib marka lagu daro peroxidase-ka-hortagga ka-hortagga aadanaha ee IgG, midabka waxaa lagu sameeyay TMB sida substrate-ka, iyo nuugista OD qiimaha waxaa lagu qiyaasaa qalab isku dheelitiran enzyme si loo go'aamiyo joogitaanka ama maqnaanshaha unugyada difaaca gaarka ah ee H. pylori ee serum ama plasma.