Enterovirus 71(EV71) IgM ELISA Kit

Enterovirus 71(EV71) Qalabka IgM ELISA Sawirka

Sharaxaad Gaaban:

Enterovirus 71 IgM (EV71-IgM) Xirmada ELISA waa baaritaanka difaaca jirka ee ku xiran enzyme-ka ee loogu talagalay ogaanshaha tayada ee unugyada difaaca jirka ee fasalka IgM ee Enterovirus 71 ee serum ama balasmaha.Waxaa loogu talagalay in lagu isticmaalo shaybaarada kiliinikada si loo ogaado iyo maareynta bukaanada la xiriira caabuqa Enterovirus 71.

Human enterovirus 71 (EV71), xubinta ugu cusub ee Enteroviridae, waa sababta caadiga ah ee cudurada gacanta, cagaha iyo afka (HFMD) waxaana mararka qaarkood lala xiriiriyaa cudurada habdhiska dhexe ee daran.Caadiyan infekshanku wuxuu kor u kacaa bilaha Juun iyo Luulyo.Caabuqa EV71 wuxuu noqon karaa labadaba asymptomatic ama wuxuu keeni karaa shuban iyo finan.Carruurta ka yar shanta sano waxay halis sare ugu jiraan inay qaadaan infekshin halis ah marka loo eego dadka waaweyn iyo carruurta waaweyn.

EV71 waxa lagu kala qaadaa qof ka qof si toos ah ula taabto iyo wasakhaynta gacmaha ama walxaha saxarada buka.Dheecaanka sanka iyo cunaha, candhuufta ama dareeraha ka soo baxa nabarrada ayaa sidoo kale faafin kara fayraska.

EV71 waa la go'doomin karaa waxaana lagu ogaan karaa dhuunta iyo saxarada iyadoo la isticmaalayo baaritaanka PCR.Fayraska RNA ayaa laga helay dareeraha maqaarka maqaarka, dhiigga iyo kaadida.Baaritaan hore oo sahlan ayaa lagu gaari karaa iyadoo la adeegsanayo EV71-specific serological assays, oo ay ku jiraan baaritaanno u gaar ah IgM antibody.

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Qalabkani waxa uu ogaanayaa Enterovirus 71 IgM antibody (EV71-IgM) ee serum bini'aadamka ama muunadaha balasmaha, xariijimaha polystyrene microwell ayaa horay loogu dahaadhay unugyada difaaca jirka ee loogu talagalay borotiinka immunoglobulin M (silsiladda anti-μ) ka dib marka ugu horeysa ee lagu daro shaybaarada serum ama balasmaha si loo noqdo la baaray, unugyada difaaca jirka ee IgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (ay ku jiraan unugyada difaaca IgG ee gaarka ah) ayaa laga saarayaa iyadoo la dhaqay.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xidhan ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca jirka ee EV71 IgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku dhafan waxay kicisaa falcelinta horumarinta midabka. si aad u abuurto walaxda buluuga ah, ku dar 50μL ee Stop Solution, oo huruud isu rogo.Joogitaanka nuugista EV71-IgM antibody ee muunada waxaa go'aamiyay akhristaha microplate.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	Habka qabsashada
Shahaado	CE
Tijaabada	Serum / plasma
Tilmaamid	48T / 96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12 bilood