Enterovirus 71(EV71) IgG ELISA Kit

Enterovirus 71(EV71) Qalabka IgG ELISA Sawirka

Sharaxaad Gaaban:

Human Enterovirus 71 IgG ELISA Kit waa baaritaanka difaaca jirka ee ku xiran enzyme-ka ee loogu talagalay ogaanshaha tayada ee unugyada difaaca jirka ee fasalka IgG ee Enterovirus 71 ee serum ama balasmaha.Waxaa loogu talagalay in lagu isticmaalo shaybaarada kiliinikada si loo ogaado iyo maareynta bukaanada la xiriira caabuqa Enterovirus 71.

Astaanta caafimaad ee ugu weyn ee caabuqa EV71 waa cudurka gacanta, cagaha iyo afka.Qaar ka mid ah carruurta qaba caabuqa EV71 waxay qabaan faryngitis herpetic, iyo xaaladaha daran, fayraska encephalitis, fayraska encephalomyelitis, bararka sambabada iyo dhiigbax sambabada.

Baadhitaanka gaarka ah ee caabuqa EV71 wuxuu ku tiirsan yahay ogaanshaha EV71-RNA, EV71-IgM iyo EV71-IgG unugyada difaaca jirka ee serum, ama EV71-RNA ee muunadaha suufka.

EV71-IgM waa tilmaame muhiim ah oo ah infekshanka aasaasiga ah ama dhawaanahan waxayna fududeeyaa ogaanshaha hore iyo daaweynta caabuqa EV71.EV71-IgG waa tilmaame muhiim ah oo lagu ogaanayo kala duwanaanshaha caabuqa waxaana loo isticmaali karaa baaritaanka cudurrada faafa iyo qiimeynta tallaalka.Waxa loo isticmaali karaa baadhista cudurrada faafa iyo qiimaynta saamaynta tallaalka.Isbeddelka titers antibody ee labadaba soo kabsaday iyo dhiigga degdega ah ayaa sidoo kale go'aamin kara caabuqa EV71, iyo haddii titer of serum antibody uu ku korodho joomatari ahaan 4 muddada soo kabsaday marka la barbar dhigo xilliga ba'an, markaas waxaa lagu xukumi karaa sida EV71 soo bandhigaysa caabuq.

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Mabda'a

Qalabkani waxa uu ku ogaanayaa Enterovirus 71 IgG antibody (EV71-IgG) ee serum bini'aadamka ama muunadaha balaasmaha, xariijimaha microwell polystyrene ayaa horay loogu dahaadhay Enterovirus 71 antigen.Ka dib marka ugu horeysa ee lagu daro shaybaarada serum ama balasmaha si loo baaro, unugyada difaaca gaarka ah ee u dhigma (EV71-IgG-Ab & qaar IgM-Ab) ee ku jira shaybaarada bukaanku waxay ku xidhaan antigens marxaladda adag, iyo qaybaha kale ee aan xidhnayn ayaa laga saari doonaa iyadoo la dhaqay.Talaabada labaad, HRP (horseradish peroxidase) -isku-dhafan ee ka-hortagga aadanaha ee IgG ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca jirka ee EV71 IgG.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (EV71 Ag) - (EV71-IgG) - (ka-hortagga bini'aadamka IgG-HRP) difaaca jirka, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku-dhafka ayaa kicinaya falcelinta horumarinta midabka. si aad u abuurto walaxda buluuga ah, ku dar 50μL ee Stop Solution, oo huruud isu rogo.Joogitaanka nuugista unugyada difaaca jirka ee EV71-IgG ee muunada waxaa go'aamiyay akhristaha microplate.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	Habka aan tooska ahayn
Shahaado	CE
Tijaabada	Serum / plasma
Tilmaamid	48T / 96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12 bilood