Enterovirus 71(EV71) Qalabka Tijaabada Degdega ah ee IgM

Enterovirus 71(EV71) Qalabka Tijaabada Degdega ah ee IgM waa xariijin tayo leh oo ku salaysan immunoassay si loo ogaado unugyada difaaca jirka ee EV71 IgM ee serumka bini'aadamka iyo xididdada dhiigga oo dhan.Cajalka imtixaanku wuxuu ka kooban yahay 1) suuf isku xidhan oo midab-burgundy ah oo ka kooban EV71 monoclonal antibody-EV71 antigen complex.Isku dhafka dahabka Colloid;2) xariijinta xuubka nitrocellulose oo ka kooban xariiq tijaabo ah (T line) iyo xariiqa kontoroolka (C line) .T ayaa horay loogu dahaadhay kahortaga bini'aadamka IgM antibody (μ-chain).iyo C-line waxaa horay loogu dahaadhay EV71 polyclonal antibody.

Marka shaybaarada la farsameeyo oo si fiican loogu daro muunada, muunada waxa la nuugaya aaladda ficilka xididka.Haddii EV71 IgM ay ku jirto muunada, waxay ku xidhmi doontaa EV71 monoclonal antibody-EV71 antigen conjugates.Immunocomplex-ka ayaa markaa lagu falceliyaa reagenti hore loo dahaadhay oo ku yaal khadka T, taas oo muujinaysa in ka hortagga EV71 IgM uu yahay mid togan.Si loogu adeego sida habraaca kontoroolka, xariiq midabaysan ayaa had iyo jeer ka soo muuqan doona gobolka xariiqda kantaroolka taasoo tusinaysa in mugga muunada saxda ah lagu daray oo xuubka dillaacay uu dhacay.