Qalabka Toxoplasma IgG ELISA

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Toxoplasma IgG ELISA Kit waa shaybaar immunosorbent-ku-xiran enzyme-ka oo loogu talagalay ogaanshaha tayada ee unugyada difaaca jirka ee fasalka IgG ee Toxoplasma ee serum ama balasmaha.Waxaa loogu talagalay in lagu isticmaalo shaybaarada kiliinikada si loo ogaado iyo maaraynta bukaanada la xiriira caabuqa Toxoplasma.Guud ahaan, kor u kaca unugyada difaaca jirka ee Toxoplasma IgM ayaa marka hore soo muuqda ka dib caabuqa Toxoplasma, kaas oo la ogaan karo qiyaastii 6 maalmood ka dib caabuqa, ka dibna si tartiib ah u kaca oo sii jiraya dhowr toddobaad ilaa bilo, ugu dambeyntiina tartiib tartiib tartiib tartiib ah ilaa uu ka ba'o.Antibody IgG togan ayaa tilmaamaya infekshankii hore ee Toxoplasma gondii, badanaa dabadheeraad ah, laakiin sidoo kale wuxuu noqon karaa mid daran.Baaritaanka ugu horreeya ee unugyada difaaca jirka ee IgG ka dib, isla baaritaankaas ayaa soo noqnoqda 2-3 toddobaad ka dib haddii ay jirto koror 4-laab ah ama ka badan oo ah awoodda unugyada difaaca jirka, baaritaan dheeraad ah ayaa loo baahan yahay.

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Mabda'a

Qalabkani waxa uu ogaanayaa Toxoplasma lgG antibody (TOX-lg) ee serum bini'aadamka ama shaybaarada balaasmaha, xariijimaha microwell polystyrene ayaa horay loogu dahaadhay Toxoplasma antigen.Ka dib marka ugu horeysa ee lagu daro shaybaarada serum ama balasmaha si loo baaro, unugyada difaaca gaarka ah ee u dhigma (TOX-lgG-Ab & qaar ka mid ah lgM-Ab) ee ku jira shaybaarada bukaanku waxay ku xidhaan antigens marxaladda adag, iyo qaybaha kale ee aan xidhnayn ayaa laga saari doonaa iyadoo la dhaqay.Talaabada labaad, HRP(horseradish peroxidase) -LgG-ga lidka ku ah bini'aadamka ee isku xidhan ayaa si gaar ah uga falcelin doona oo keliya TOX lgG unugyada difaaca jirka.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (TOX Ag) - (TOX-lgG) - (LgG-HRP-ka-hortagga bini'aadamka) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP oo ku xiran isku-dhafka ayaa kicinaya falcelinta horumarinta midabka. si loo soo saaro walaxda buluuga ah, ku dar 50 µ I ee Stop Solution, oo huruud isu rogo.Joogitaanka nuugista ka hortagga TOX-lgG ee muunada waxaa go'aamiyay akhristaha microplate.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	Habka aan tooska ahayn
Shahaado	NMPA
Tijaabada	Serum / plasma
Tilmaamid	48T / 96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12 bilood