Qalabka Cytomegalovirus Human IgM ELISA

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Human Cytomegalovirus IgM (HCMV-IgM) ELISA Kit waa enzyme-ku-xiran immunosorbent asay ee loogu talagalay ogaanshaha tayada ee unugyada difaaca fasalka IgM ee Human Cytomegalovirus ee serum aadanaha ama balasmaha.Waxaa loogu talagalay in lagu isticmaalo shaybaarada kiliinikada si loo baaro loona maareeyo bukaanada la xiriira infekshinka Human Cytomegalovirus.HCMV waa mid ka mid ah sababaha ugu muhiimsan ee cilladaha ku dhasha bini'aadamka.Caabuqyada asaasiga ah ama kuwa cusub ee soo noqnoqda ee haweenka uurka leh waxay keeni karaan infekshanka intrauterine ama mindhicirka ee dhallaanka cusub.80% uurjiifka uu ku dhacay HCMV inta lagu guda jiro uurka aasaasiga ah waxay yeelan karaan calaamado ay ka mid yihiin dib u dhac maskaxeed, aragga oo daciifa, iyo maqal la'aan.Infekshanka HCMV ee aasaasiga ah wuxuu leeyahay cawaaqib halis ah oo xitaa nafta halis gelinaya bukaannada difaaca jirkoodu hooseeyo, iyo heerka dhimashada ee pneumonia interstitial sababtoo ah caabuqa HCMV ee ku-tallaalidda dhuuxa lafta ayaa ah ilaa 75%.Dhawaan, HCMV ayaa la ogaaday inay tahay mid ka mid ah ilaha caabuqyada fursada ah ee bukaanka AIDS-ka.

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Qalabkani waxa uu ku ogaanayaa Human Cytomegalovirus IgM antibody (HCMV-IgM) ee serum bini'aadamka ama muunadaha balasmaha, xariijimaha polystyrene microwell ayaa horay loogu dahaadhay unugyada difaaca jirka ee ku wajahan borotiinka Immunoglobulin M borotiinka (silsiladda lidka-µ).Ka dib marka ugu horeysa ee lagu daro shaybaarka serum ama balasmaha si loo baaro, unugyada difaaca jirka ee IgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (oo ay ku jiraan unugyada difaaca IgG ee gaarka ah) ayaa laga saari doonaa dhaqid.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xidhan ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca jirka ee HCMV IgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-µ) -(HCMV-lgM) - (HCMV Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku dhafan waxay kicisaa falcelinta horumarinta midabka dhali walaxda buluuga ah, ku dar 50 µI ee Stop Solution, oo huruud isu beddel.Joogitaanka nuugista HCMV-IgM antibody ee muunada waxaa go'aamiyay akhristaha microplate.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	Habka qabsashada
Shahaado	NMPA
Tijaabada	Serum / plasma
Tilmaamid	48T / 96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12 bilood