Mycoplasma Pneumonia IgM ELISA Kit

Qalabkani waxa uu ogaanayaa Mycoplasma Pneumoniae IgM (MP-IgM) ee serum bini'aadamka ama muunadaha plasma, xariijimaha polystyrene microwell ayaa horay loogu dahaadhay unugyada difaaca jirka ee loogu talagalay borotiinnada Immunoglobulin M (silsiladda anti-μ) ka dib marka ugu horeysa ee lagu daro shaybaarka serum ama balaasmaha si loo baaro , unugyada difaaca jirka ee IgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (oo ay ku jiraan unugyada difaaca IgG ee gaarka ah) ayaa laga saarayaa iyadoo la dhaqay.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xidhan ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca MP IgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku-dhafka ayaa kicinaya falcelinta horumarinta midabka. si aad u abuurto walaxda buluuga ah, ku dar 50μl Stop Solution, oo huruud isu rogo.Joogitaanka nuugista antibody MP-IgM ee muunada waxaa go'aamiyay akhristaha microplate.