Fayraska Jadeecada (MV) IgG ELISA Kit

Qalabkani waxa uu ku ogaanayaa fayraska jadeecada IgG antibody (MV-IgG) ee serum bini'aadamka ama muunadaha balasmaha, xariijimaha polystyrene microwell ayaa horay loogu dahaadhay antigen fayraska jadeecada.Ka dib marka ugu horeysa ee lagu daro shaybaarada serum ama balasmaha si loo baaro, unugyada difaaca gaarka ah ee u dhigma (MV-IgG-Ab & qaar IgM-Ab) ee ku jira shaybaarada bukaanku waxay ku xidhaan antigens marxaladda adag, iyo qaybaha kale ee aan xidhnayn ayaa laga saarayaa iyadoo la dhaqay.Talaabada labaad, HRP (horseradish peroxidase) -la-dagaalanka bini'aadamka IgG ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca jirka ee MV IgG.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (MV Ag) - (MV-IgG) - (ka-hortagga bini'aadamka IgG-HRP) difaaca jirka, ka dib markii saxanka la dhaqo, substrate TMB ayaa lagu daray horumarinta midabka, iyo HRP oo ku xiran isku-dhafka ayaa kicinaya falcelinta horumarinta midabka. si loo dhaliyo walaxda buluuga ah, ku dar 50μl ee Stop Solution, oo huruud noqda. Joogitaanka nuugista antibody MV-IgG ee muunada waxaa go'aamiyay akhristaha microplate.