Herpes Simplex virus IgM ELISA Kit

Qalabkani waxa uu ku ogaanayaa Herpes Simplex Virus I lgM antibody (HSV1-lgM) ee serum bini'aadamka ama muunadaha balasmaha, xariijimaha polystyrene microwell waxaa horay loogu dahaadhay unugyada difaaca jirka ee ku wajahan borotiinnada Immunoglobulin M (silsiladda lidka-µ) ee aadanaha.Ka dib marka ugu horeysa ee lagu daro shaybaarka serum ama balasmaha si loo baaro, unugyada unugyada unugyada lgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (oo ay ku jiraan unugyada difaaca ee lgG gaar ah) ayaa laga saarayaa iyadoo la dhaqay.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xidhan ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca jirka ee HSV1 lgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku dhafan waxay kicisaa falcelinta horumarinta midabka dhali walaxda buluuga ah, ku dar 50 µ I of Stop Solution, oo huruud isu beddel.Joogitaanka nuugista HSV1-lgM antibody ee muunada waxaa go'aamiyay akhristaha microplate.