Qalabka Herpes Simplex II IgM ELISA

Qalabkan waxa lagu ogaanayaa fayraska Herpes Simplex II IgM antibody (HSV2-IgM) ee serum bini'aadamka ama muunadaha balasmaha, xariijimaha polystyrene microwell ayaa horay loogu dahaadhay unugyada difaaca jirka ee ku wajahan borotiinka Immunoglobulin M borotiinka (silsiladda lidka-µ).Ka dib marka ugu horeysa ee lagu daro shaybaarka serum ama balasmaha si loo baaro, unugyada difaaca jirka ee IgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (oo ay ku jiraan unugyada difaaca IgG ee gaarka ah) ayaa laga saari doonaa dhaqid.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xidhan ayaa si gaar ah uga falcelin doona oo keliya unugyada difaaca jirka ee HSV2 IgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-µ)-(HSV2-lgM)-(HSV2-lgM)-(HSV2 Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku dhafan waxay kicisaa falcelinta horumarinta midabka dhali walaxda buluuga ah, ku dar 50 µ I of Stop Solution, oo huruud isu beddel.Joogitaanka nuugista HSV2-IgM antibody ee muunada waxaa go'aamiyay akhristaha microplate.