Hepatitis D fayraska IgM ELISA Kit

Qalabka Cagaarshowga D ee IgM ELISA Sawirka

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Fayraska cagaarshow D IgM ELISA Kit waa shaybaar immunosorbent ku xiran enzyme-ka oo loogu talagalay ogaanshaha tayada ee unugyada difaaca ee heerka IgM ee fayraska cagaarshowga D ee serum ama balasmaha.Waxaa loogu talagalay in lagu isticmaalo shaybaarada kiliinikada si loo baaro loona maareeyo bukaanada la xiriira caabuqa cagaarshowga D.Ka-hortagga HDV-IgM togan wuxuu muujinayaa infekshan HDV ah oo degdeg ah ama dhawaan;Infekshanka wadajirka ah ee HDV/HBV wuxuu la xiriiraa ka-hortagga HDV-IgM ee ku-meel-gaadhka ah;caabuq is dulsaaran waxa uu la xidhiidhaa anti-HDV-IgM togan oo joogto ah;sidaas darteed, tijaabada anti-HDV-IgM waxay leedahay qiime ogaanshaha oo muhiim ah.Ka-hortagga HDV-IgM waxay kala saari karaan infekshannada dhawaanahan ama kuwii hore, caabuqyada wadajirka ah iyo infekshannada is-daba-jooga, iyo titer-yada sare ee joogtada ah ee ka-hortagga HDV-IgG iyo IgM ayaa si isdaba-joog ah isu bedbeddela, kuwaas oo ah tilmaamayaasha muhiimka ah ee caabuqyada HDV ee daba-dheeraaday, markaa ogaanshaha ka-hortagga- HDV-IgM waxay muhiim u tahay ogaanshaha iyo odoroska infekshannada HDV.

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Qalabkan waxa lagu ogaanayaa fayraska cagaarshow D ee IgM antibody (HDV-IgM) ee serum bini aadamka ama muunadaha balasmaha, xariijimaha polystyrene microwell ayaa hore loogu dahaadhay unugyada difaaca jirka ee ku wajahan borotiinnada Immunoglobulin M ee aadanaha (silsiladda lidka μ).Ka dib marka ugu horeysa ee lagu daro shaybaarka serum ama balasmaha si loo baaro, unugyada difaaca jirka ee IgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (oo ay ku jiraan unugyada difaaca IgG ee gaarka ah) ayaa laga saari doonaa dhaqid.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xiran ayaa si gaar ah uga falcelin doona kaliya unugyada difaaca jirka ee HDV IgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku dhafan waxay kicisaa falcelinta horumarinta midabka dhali walaxda buluuga ah, ku dar 50μl ee Stop Solution, oo huruud isu beddel.Joogitaanka nuugista antibody HDV-IgM ee muunada waxaa go'aamiyay akhristaha microplate.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	Habka qabsashada
Shahaado	NMPA
Tijaabada	Serum / plasma
Tilmaamid	48T / 96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12 bilood