Hepatitis E fayraska IgM ELISA Kit

Qalabka Cagaarshowga E ee IgM ELISA Qalabka Sawirka leh

Sharaxaad Gaaban:

Cagaarshowga E fayraska IgM Test Cassette waxa loo isticmaalaa in si tayo leh loo ogaado Cagaarshowga E Virus IgM unugyada difaaca jirka ee serum, plasma (EDTA, heparin, sodium citrate) ama dhiiga oo dhan (EDTA, heparin, sodium citrate).Baaritaanka waa in loo isticmaalo sidii gargaar lagu ogaan lahaa cagaarshowga fayraska, kaas oo uu keeno Fayraska Cagaarshowga E.

Fayraska Cagaarshowga E (HEV) waa fayraska RNA ee aan bakhshad lahayn, hal xarig ah oo ugu horrayn lagu kala qaado dariiqa saxarada- afka, dhiig ku shubidda iyo suurtogalnimada hooyada- uurjiifka.Caabuqa HEV wuxuu keenaa cagaarshow ba'an iyo cudur faafa oo ba'an wuxuuna keenaa cudurro beerka oo ba'an ama kuwo hoose oo la mid ah Cagaarshowga A. Halka ay jiraan afar genotypes waaweyn ee HEV, waxaa jira hal serotype oo keliya.

Caabuqa HEV ee bini'aadamka wuxuu soo saaraa IgM, IgA iyo IgG unugyada difaaca jirka.HEV-IgM iyo HEV-IgA positivity waa calaamad caabuq ba'an ama dhawaanahan.Haddii anti-HEV-IgM iyo ka-hortagga HEV-IgA ay u wanaagsan yihiin hal ama labadaba, waxay muujinayaan caabuqa HEV ee dhowaanta.Jiritaanka caabuqa HEV ee dhawaanta, oo ay weheliso shaqada beerka, ayaa loo isticmaali karaa in lagu go'aamiyo in infekshanku yahay mid degdeg ah ama mid dhow.Jiritaanka caabuqa HEV ee beerka ayaa loo isticmaali karaa si loo go'aamiyo in cudurku yahay cagaarshow E ba'an ama ka soo kabashada cagaarshowga degdega ah E.

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Mabda'a

Qalabkani waxa uu ku ogaanayaa fayraska cagaarshow E IgM antibody (HEV-IgM) ee serum bini'aadamka ama shaybaarrada balaasmaha, xariijimaha polystyrene microwell ayaa horay loogu dahaadhay unugyada difaaca jirka ee ku wajahan borotiinnada Immunoglobulin M ee aadanaha (silsiladda lidka μ).Ka dib marka ugu horeysa ee lagu daro shaybaarka serum ama balasmaha si loo baaro, unugyada difaaca jirka ee IgM ee muunada waa la qabsan karaa, iyo qaybaha kale ee aan xidhnayn (oo ay ku jiraan unugyada difaaca IgG ee gaarka ah) ayaa laga saari doonaa dhaqid.Talaabada labaad, HRP (horseradish peroxidase) - antigens-ka isku xiran ayaa si gaar ah uga falcelinaya oo keliya unugyada difaaca jirka ee HEV IgM.Ka dib marka la dhaqo si meesha looga saaro HRP-conjugate ee aan xidhnayn, xalalka koromojiinka ayaa lagu daraa ceelasha.Joogitaanka (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, ka dib markii saxanka la dhaqo, substrate-ka TMB ayaa lagu daray horumarinta midabka, iyo HRP ee ku xiran isku dhafan waxay kicisaa falcelinta horumarinta midabka dhali walaxda buluuga ah, ku dar 50μl ee Stop Solution, oo huruud isu beddel.Joogitaanka nuugista ka hortagga HEV-IgM ee muunada waxaa go'aamiyay akhristaha microplate.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	Habka qabsashada
Shahaado	CE
Tijaabada	Serum / plasma
Tilmaamid	48T / 96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12 bilood