Ka-hortagga ugxan-sidaha (AO) Qalabka ELISA ee ka hortagga unugga

Qalabkani waxa uu ku ogaanayaa unugyada difaaca-ovarian-ka (IgG) ee muunadaha serum ee bini'aadamka ee ku salaysan habka aan tooska ahayn, oo leh antigens xuub ugxan-sifeed oo la safeeyey oo loo isticmaalo dahaadhka ka horreeya microwells.

Habka shaybaadhku waxa uu ka bilaabmaa in lagu daro muunada serum ceelasha falcelinta antigen-precoated si loogu tallaalo. Haddii unugyada ka hortagga ugxan-sidaha ay ku jiraan muunada, waxay si gaar ah ugu xidhi doonaan antigens-ka xuubka ugxan-sidaha hore loo dahaadhay ee microwells, iyaga oo samaynaya dhismayaal antigen-antibody deggan. Qaybaha aan xidhnayn ayaa markaa laga saaraa si loo hubiyo saxnaanta ogaanshaha.

Marka xigta, malayga peroxidase (HRP) -ku calaamadsan mouse ka-hortagga unugyada IgG-ga bini'aadamka ayaa lagu daraa ceelasha. Kahortagga labaad ka dib, unugyadan-ka-hortagga enzyme-ku-calaamadeysan waxay si gaar ah ugu xidhaan unugyada-ka-hortagga ugxan-sidaha ee ka-hortagga unugyada antigen-antibody-ka ee jira, iyaga oo samaynaya isku-xidhka difaaca oo dhammaystiran "antigen-antibody-enzyme".

Ugu dambeyntii, xalka substrate TMB ayaa lagu daraa. HRP-ga kakan waxa uu kiciyaa falcelinta kiimikaad ee TMB, iyada oo soo saarta isbeddel muuqda oo midab leh. Nuugista (Qiimaha A) ee xalka falcelinta waxaa lagu cabbiraa iyadoo la adeegsanayo akhristaha microplate, joogitaanka ama maqnaanshaha unugyada difaaca jirka ee shaybaarka ayaa lagu go'aamiyaa iyadoo lagu saleynayo natiijada nuugista.