Kahortagga bini'aadamka Chorionic Gonadotropin (HCG) Qalabka ELISA ee Kahortagga Jirka

Kahortagga Bani'aadamka Chorionic Gonadotropin (HCG) Qalabka ELISA ee Kahortagga Jirka

Sharaxaad Gaaban:

Xirmadan waxaa loogu talagalay in lagu ogaado tayada jirka ee ka-hortagga-hormoonka gonadotropin-ka-hortagga aadanaha (HCG-Ab) ee serumka aadanaha.

HCG-Ab waa autoantibody oo ka mid ah sababaha ugu waaweyn ee dhalmo la'aanta difaaca jirka. Human chorionic gonadotropin (HCG), hormoon xaamilo-gaar ah oo qarsoodi ah syncytiotrophoblasts, ugu horreyntii waxay u shaqeysaa si kor loogu qaado horumarinta luteum corpus uurka leh iyo dheecaanka hormoonnada steroid. Waxa ay door muhiim ah ka ciyaartaa ilaalinta uurka hore iyo ka hortagga diidmada hooyada ee uurjiifka, iyada oo u adeegta sida hoormoonka muhiimka ah ee joogteynta uurka hore ee uurjiifka.

HCG-Ab waxaa inta badan la soo saaraa labaad ka dib dhicis ama irbado HCG. Qiyaastii 40% dadka leh taariikhda dhicinta ayaa laga helay HCG-Ab. Marka HCG-Ab ay ku xidho HCG, waxay xannibtaa goobta firfircoon ee HCG waxayna joojisaa hawlaheeda jireed, taasoo ka dhigaysa uurka mid aan sii waarin oo si fudud u horseeda dhicis caadi ah ama soo noqnoqda, taas oo keenta dhalmo la'aan. Saameynteeda dhalmo la'aanta-sababteeda waxaa lagu taageeray caddaynta sida dhibaatooyinka dib-u-uurka ka dib irbado HCG iyo saamaynta ka hortagga uur-qaadidda ee tallaalka HCG.

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Mabda'a

Qalabkani waxa uu ogaanayaa unugyada ka-hortagga chorionic gonadotropin-ka bini'aadamka ee shaybaarada serum bini'aadamka oo ku salaysan habka aan tooska ahayn, oo leh antigens chorionic gonadotropin bini'aadam oo la nadiifiyey oo loo isticmaalo ka-hor-istaagga microwells.

Habka shaybaadhku waxa uu ka bilaabmaa in lagu daro muunada serum ceelasha falcelinta antigen-precoated si loogu tallaalo. Haddii unugyada ka-hortagga gonadotropin-ka-hortagga bini'aadamka ay ku jiraan muunada, waxay si gaar ah ugu xidhi doonaan antigens-ka hore loo dahaadhay ee microwells, samaynta dhismooyin antigen-antibody deggan.

Marka xigta, isku-dhafka enzymes ayaa lagu daraa. Ka dib markii labaad, Enzymes conjugates waxay ku xidhmaan dhismooyinka antigen-antibody ee jira. Marka substrate-ka TMB la soo bandhigo, falcelinta midabka ayaa hoos timaada catalysis of enzyme. Ugu dambeyntii, akhristaha microplate wuxuu cabbiraa nuugista (qiimaha A), kaas oo loo isticmaalo si loo go'aamiyo joogitaanka unugyada difaaca jirka ee chorionic gonadotropin ee muunada.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	DadbanHabka
Shahaado	NMPA
Tijaabada	Serum / plasma
Tilmaamid	48T /96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12bilo