Xirmada ELISA ee ka hortagga endometrial (EM).

Anti-Endometrial (EM) Qalabka ELISA ee Kahortagga Jirka

Sharaxaad Gaaban:

Xirmadan waxaa loo isticmaalaa in vitro lagu ogaado tayada unugyada anti-endometrial (EmAb) ee serumka aadanaha.

EmAb waa autoantibody bartilmaameedsanaya endometrium, kicinaya jawaabaha difaaca. Waa calaamade antibody for endometriosis oo la xidhiidha dhicis dumarka iyo dhalmo la'aanta. Warbixinadu waxay muujinayaan 37%-50% bukaanada qaba dhalmo la'aan, dhicin ama endometriosis inay yihiin EmAb-positive; heerka wuxuu gaarayaa 24%-61% dumarka ka dib ilmo iska soo ridid macmal ah.

EmAb waxay ku xidhan tahay antigens-ka endometrial, waxyeelo gaadhsiisa endometrium iyada oo loo sii marayo hawlgelinta dhamaystirka iyo qoritaanka unugyada difaaca, wax u dhimaya embriyaha abuurka iyo sababa dhicin. Inta badan waxay la mid tahay endometriosis, iyada oo heerka lagu ogaanayo 70%-80% bukaannada noocaas ah. Qalabkani wuxuu caawiyaa in la baaro endometriosis, la socdo saameynta daaweynta, iyo hagaajinta natiijooyinka dhalmo la'aanta la xiriirta.

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Qalabkani waxa uu ku ogaanayaa unugyada ka-hortagga endometrial-ka (IgG) ee muunadaha serum ee bini-aadmiga oo ku salaysan habka aan tooska ahayn, oo leh antigens xuubka endometrial oo la safeeyey oo loo isticmaalo dahaadhka ka hor microwells.

Habka baadhistu waxa uu ku bilaabmayaa in lagu daro muunada serum ceelasha falcelinta antigen-precoated si loogu tallaalo. Haddii unugyada anti-endometrial ay ku jiraan muunada, waxay si gaar ah ugu xidhi doonaan antigen-ka hore loo dahaadhay ee endometrial antigens ee microwells, samaynta dhismooyin antigen-antibody deggan. Ka dib markii laga saaro qaybaha aan xidhnayn iyada oo loo marayo dhaqid si looga fogaado faragelinta, malayga peroxidase-ku calaamadeeyay jiirka ka hortagga-aadmiga IgG-ga ayaa lagu daraa.

Ka-hortagga unugyada ka-hortagga-antigen-ka ee jira ka dib, unugyadan-ka-hortagga-antigen-ka ee ku suntan enzyme-ka. Marka substrate TMB lagu daro, falcelinta midabka ayaa hoos timaada catalysis of enzyme. Ugu dambeyntii, akhristaha microplate wuxuu cabbiraa nuugista (qiimaha A), kaas oo loo isticmaalo si loo go'aamiyo joogitaanka unugyada anti-endometrial (IgG) ee muunadda.

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Mabda'a	Baaritaanka difaaca jirka ee Enzyme ayaa ku xiran
Nooca	DadbanHabka
Shahaado	NMPA
Tijaabada	Serum / plasma
Tilmaamid	48T /96T
Heerkulka kaydinta	2-8℃
Nolosha shelf	12bilo